PLoS Negl Trop Dis. 2021 Apr 16;15(4):e0009290. doi: 10.1371/journal.pntd.0009290. eCollection 2021 Apr.ABSTRACTSince introduction into Brazil in 2014, chikungunya virus (CHIKV) has presented sustained transmission, although much is unknown about its circulation in the midwestern states. Here, we analyze 24 novel partial and near complete CHIKV genomes from Cuiaba, an urban metropolis located in the Brazilian midwestern state of Mato Grosso (MT). Nanopore technology was used for sequencing CHIKV complete genomes.